Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

The polymerase chain reaction (PCR) is a molecular method that is employed for in vitro amplification of a specific region in a strand of deoxyribonucleic acid (DNA). Multiplex PCR involves the ...

Researchers from the Zhang Liye Laboratory have developed a groundbreaking tool that revolutionizes the way researchers ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...